Conduit Lab Microtubule regulation in multi-cellular animals Department of Zoology, University of Cambridge, UK and Institut Jacques Monod, Université de Paris, France

Dr. Paul T Conduit

Group Leader, Sir Henry Dale Fellow

 

After an undergraduate degree in Biological Sciences at the University of Birmingham, I joined Dr. John Kilmartin's lab as a Research Assistant in 2004 at the LMB/MRC in Cambridge. I learnt the fundamentals of lab work and good scientific practice and after two constructive years I started a PhD with Prof. Jordan Raff at the Gurdon Institute in Cambridge. During my PhD I discovered a surprising mechanism of centrosome assembly and showed how this influenced centrosome size and inheritance in neural progenitor cells. In 2010 I completed my PhD and moved with Prof. Raff as a post-doc to the Sir William Dunn School of Pathology in Oxford. Here, I helped elucidate the molecular details of centrosome assembly and studied how centrosomes are able to nucleate large numbers of microtubules during mitosis. In 2015 I moved back to Cambridge to the Department of Zoology to set up my own lab studying microtubule nucleation from both centrosomes and from other microtubule organising centres in the cell. In 2020, I was awarded a CNRS CRCN position and a Chaire d'Excellence position from the Université de Paris to move my lab to the Institut Jacques Monod in Paris.

 

Email: ptc29@cam.ac.uk

Office Phone: 0044 1223 334471

Paul Brooks

Postdoc, ex PhD Student

 

After completing my degree in Cell Physiology and Pharmacology in 2012, I began work at Cambridge in the lab of Matthias Landgraf, where I was working with Drosophila as a model system. Our research focus was to establish how the nervous system can homeostatically maintain a physiologically preferable activity level (output), in response to increased stimulus from the brain (input).

 

My current area of research focuses on how the microtubule cytoskeleton is formed and organised in neurons, again using Drosophila as a model organism. My PhD was based on understanding the role of Patronin within neurons. I completed my PhD within the Conduit lab in 2019 and I am now finishing off my work as a postdoc.

 

Email: pb604@cam.ac.uk

Office Phone: 0044 1223 334449

Corinne Tovey

Postdoc, ex PhD Student

 

I completed my undergraduate degree in Biological Sciences in 2015 and was awarded a place on the BBSRC DTP in Cambridge. I did a rotation in the Conduit lab from January 2016, and then joined as a PhD student in April 2016. My PhD focused on investigating γ-TuRC composition and whether or not this varies in different cell types in Drosophila. I published my PhD work in 2018 and passed my Viva in 2019. I am continuing as a postdoc within the lab to study how g-TuRC binding and activation is regulated.

 

Email: cap75@cam.ac.uk

Office Phone: 0044 1223 334449

 

 

 

Angela Trowsdale

Senior technician

 

After completing my PhD at the University of Sussex I worked as a PDRA in the Department of Genetics at the University of Cambridge. I joined the Conduit lab as a senior technician in 2017 and, while supporting the day-to-day running of the lab, I am also involved in projects to understand how centrosome duplication is regulated and how g-TuRC activity is regulated.

 

 

at821@cam.ac.uk

Office Phone: 0044 1223 334449

 

 

Imene Bouhlel

Postdoc

 

I have always been keen to understand how Centrosomes function during cell division. I started working as a research assistant in 2008, in the lab of Valérie Doye, at Institut Jacques Monod in Paris. During this time, I studied the role of the nuclear pore complex Nup160-107 in anchoring the centrosome to the nuclear envelope in human cells and characterized the dynamics of this complex during mitosis. In 2011 I joined the lab of Anne Paoletti at Institut Curie in Paris, first as a research assistant and then as a PhD Student. During this time, I studied the role of Sfi1, an evolutionary conserved component of the centrosome, in centrosome duplication. I demonstrated that Sfi1 and its partner Centrin are essential for centrosome duplication and separation in fission yeast. In addition, I also characterized the role of Sfi1 in human cells and showed that it was required for Centrin localization at the centrioles and for ciliogenesis.  I completed my PhD in Dec. 2017 and joined the Conduit lab as a Post-doc in beginning of 2018. My current project aims at investigating a potential role of the nuclear envelope in centrosome duplication in the Drosophila embryo.

 

ib407@cam.ac.uk

Office Phone: 0044 1223 334449

 

 

 

Amrita Mukherjee

Postdoc

 

After completing my PhD in Andrew Renault’s lab at the Max Planck Institute for Developmental Biology on extracellular lipid signals in germ cell migration, I decided to focus my research on the study of cell and developmental biology of neurons. In 2015 I joined Darren William’s lab at King’s College London, where I discovered a role for mitochondria in non-apoptotic caspase activation during developmental pruning of sensory neurons. In addition, I also uncovered a number of microtubule regulators in a targeted RNAi screen for neuron remodelling. In order to gain further insight into the role of microtubules in developing and mature neurons, I joined Paul Conduit’s lab in 2018. Here, together with excellent insight and expertise of the Conduit lab, we discovered a role of the somatic Golgi in microtubule nucleation in sensory neurons. I discovered that certain microtubule regulators were differentially expressed in different neuron types and this is an ongoing project. Together with this, I established Drosophila pupal and adult sensory neurons as great model systems to study the role of microtubule regulators in developing neurons and fully mature neurons respectively. These are now projects that are waiting to be explored in greater detail.

 

am2634@cam.ac.uk

Office Phone: 0044 1223 334449

Chisato Tsuji

MPhil

 

I am working to understand how the binding between tethering proteins and g-TuRCs is regulated and whether this influences g-TuRC activity.

 

 

ct516@cam.ac.uk

Office Phone: 0044 1223 334449

 

 

 

Lab alumni

Yaiza Andres Jeske

ex MPhil student

 

Yaiza completed her MPhil in 2019 studying the role of Augmin in Drosophila neurons. She then joined the LMB-MRC in Cambridge as a PhD student.

 

 

 

 

 

Zihan (Amanda) Zhu

ex MPhil student

 

 

Amanda completed her MPhil in 2019 studying how g-TuRCs are recruited to centrosomes. She then moved to the University of Oxford for her PhD.

 

 

 

 

Alyce McClellan

BBSRC PhD rotation student

 

Alyce started in the lab as a research assistant and then spent a PhD rotation within the lab.

 

am2744@cam.ac.uk

Office Phone: 0044 1223 334449

 

 

 

Department of Zoology

University of Cambridge

Downing Street

Cambridge

CB2 3EJ